Preliminary report on PCR detection of faecal microflora in Prunella collaris
We compared the diversity of chromosomal DNA isolated from Alpine Accentor (Prunella collaris) faeces collected from various locations in high mountains of Slovakia, Austria and Switzerland in two different years (1998 and 1999). The microflora was extracted, purified and analysed without cultivation. Bacterial 16S rDNA was amplified by PCR and the amplification products analysed by RFLP analysis. We consider this genetic approach a suitable method to characterise microbial community changes, because cultivation experiments only show the presence of a limited range of live bacterial species (Enterococcus spp. and Enterobacter spp.). Our results showed that the diversity of faecal microflora varied according to the year rather than the site of sample collection. In addition, using genus-specific primers, we showed the presence of Bifidobacterium spp. in faecal samples from the Alps.
© PRUNELLA Publishers
All rights reserved. No part of this publication may be reproduced, stored in retrieval system or transmitted in any form or by any means, photocopying, elctronic, recording, etc. without the prior written permission of the publisher, PRUNELLA Publishers - RNDr. Marián Janiga, CSc., Vavra Šrobára 1524/6, 03401 Ružomberok, Slovakia, IČO: 30 525 764. Permission requests for creating new collective works, for resale, for advertising or promotional purposes, or for general distribution, should be addressed to the publisher as mentioned in the Slovak Copyright Law. Free submission of an article for publication entails the author(s) irrevocable authorization of the publisher to collect any sums or considerations for copying or reproduction payable by third parties, as mentioned in the Slovak Copyright Law. EV 3492/09.