Bacteria in the water from the snow from the area Ružomberok
Abstract
The aim of our study was to investigate the possible environmental pollution by the Mondi SCP pulp mill in Ružomberok. Bacterial communities were examined by T-RFLP analysis of the 16S rRNA gene. This gene was subjected to restriction digestion by using the restriction enzyme MspI. Terminal restriction fragments (T-RFs) from 60-924 bp were evaluated. An experimental size of fragments was compared to in silico sizes T-RF from the database of 16S rDNA sequences by using the MiCA program. Amplification and sequencing of 16 rDNA from DNA isolated from bacteria in the snow samples indicate the presence of bacteria. It was established three categories of bacteria. The first representated the dominant phyla of 5 to 25 % (Proteobacteria 19.5 %, Firmicutes 18.2 %, Actinobacteria 13.4 %, Bacteroidetes 11.1 %, and Chloroflexi 10.1 %). The second group has a relatively low percentage of bacterial phyla 1 to 5 % (Planctomycetes, Acidobacteria, Tenericutes, Planctomycetes, Acidobacteria, Tenericutes, Spirochaetes, Cyanobacteria, Deinococcus-Thermus, Gemmatimonadetes, Fusobacteria, Nitrospirae). In the last one, the percentage of bacterial phyla ranged from 0 to 1% (Defferibacteres, Chlorobi, Verrucomicrobia, Synergistes, Fibrobacteres, Thermotogae, Caldiserica, Thermodesulfobacteria).
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